PRODUCTION OF PROTEASE FROM Bacillus species USING AGRICULTURAL WASTE BY SUBMERGED FERMENTATION

Abstract

The present investigation carried out on production of protease from Bacillus species using agricultural waste by submerged fermentation. 10 different soil sample was collected from Department of Microbiology, RTMNU, Nagpur. Out of 10 soil samples four soil samples were confirmed for the production of protease enzyme from Bacillus species. The present study describes the screening of two agricultural wastes i.e. gram husk and wheat husk to enhance the production of protease enzyme by Bacillus species by submerged fermentation. The present study on the optimization of protease production with respect to incubation time and it results that protease production increased with the use of agro-industrial waste like gram husk and wheat husk. The best result was obtained by Bacillus megaterium at incubation time of 4 days (96h) yielded 0.144mg/ml protease production by using wheat husk as a substrate (agrowaste). The environmental conditions of the fermentation play a vital role in the growth and metabolic production of microbial population. Optimization of different medial components can greatly affect the enzyme production cost.